dc.contributor.author |
Van der Nest, Magrieta Aletta
|
|
dc.contributor.author |
Olsen, Andreas
|
|
dc.contributor.author |
Karlsson, M.
|
|
dc.contributor.author |
Lind, Monica
|
|
dc.contributor.author |
Dalman., K.
|
|
dc.contributor.author |
Brandström-Durling, M.
|
|
dc.contributor.author |
Elfstrand, M.
|
|
dc.contributor.author |
Wingfield, Brenda D.
|
|
dc.contributor.author |
Stenlid, J.
|
|
dc.date.accessioned |
2015-03-18T09:52:09Z |
|
dc.date.available |
2015-03-18T09:52:09Z |
|
dc.date.issued |
2014-12 |
|
dc.description.abstract |
Intersterility (IS) is thought to prevent mating compatibility between homokaryons that belong to different
species. Although IS in Heterobasidion is regulated by the genes located at the IS loci, it is not yet
known how the IS genes influence sexual compatibility and heterokaryon formation. To increase our
understanding of the molecular events underlying IS, we studied mRNA abundance changes during IS
compatible and incompatible interactions over time. The clustering of the transcripts into expression profiles,
followed by the application of Gene Ontology (GO) enrichment pathway analysis of each of the clusters,
allowed inference of biological processes participating in IS. These analyses identified events
involved in mating and sexual development (i.e., linked with IS compatibility), which included processes
associated with cell–cell adhesion and recognition, cell cycle control and signal transduction. We also
identified events potentially involved in overriding mating between individuals belonging to different
species (i.e., linked with IS incompatibility), which included reactive oxygen species (ROS) production,
responses to stress (especially to oxidative stress), signal transduction and metabolic biosynthesis. Our
findings thus enabled detection and characterization of gene expression changes associated with IS in
Heterobasidion, as well as identification of important processes and pathways associated with this phenomenon.
Overall, the results of this study increase current knowledge regarding the molecular mechanisms
underpinning IS in Heterobasidion and allowed for the establishment of a vital baseline for further
studies. |
en_ZA |
dc.description.librarian |
hb2015 |
en_ZA |
dc.description.sponsorship |
National Research Foundation, the Swedish Foundation for Strategic Research
The Swedish Research Council for Environment, Agricultural Sciences and Spatial
Planning (Uppsala Microbiomics Center). |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/yfgbi |
en_ZA |
dc.identifier.citation |
Van der Nest, MA, Olson, A, Karlsson, M, Lind, M, Dalman, K, Brandstrom-Durling, M, Elfstrand, M, Wingfield, BD & Stenlid, J 2014, 'Gene expression associated with intersterility in Heterobasidion', Fungal Genetics and Biology, vol. 73, pp. 104-119. |
en_ZA |
dc.identifier.issn |
1087-1845 (print) |
|
dc.identifier.issn |
1096-0937 (online) |
|
dc.identifier.other |
10.1016/j.fgb.2014.10.008 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/44033 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2014 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Fungal Genetics and Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Genetics and Biology, vol. 73, pp. 104-119, 2014. doi : 10.1016/j.fgb.2014.10.008 |
en_ZA |
dc.subject |
Heterobasidion occidentale |
en_ZA |
dc.subject |
Heterobasidion irregulare |
en_ZA |
dc.subject |
Mating |
en_ZA |
dc.subject |
RNA-seq |
en_ZA |
dc.subject |
Intersterility (IS) |
en_ZA |
dc.title |
Gene expression associated with intersterility in Heterobasidion |
en_ZA |
dc.type |
Postprint Article |
en_ZA |