In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-beta-estradiol analogue in breast adenocarcinoma cells

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dc.contributor.author Nkandeu, Danielle Sandra
dc.contributor.author Mqoco, Thandi Vuyelwa
dc.contributor.author Visagie, Michelle Helen
dc.contributor.author Stander, Barend Andre
dc.contributor.author Wolmarans, Elize
dc.contributor.author Cronje, Marianne J.
dc.contributor.author Joubert, Annie M.
dc.date.accessioned 2013-11-26T09:58:33Z
dc.date.available 2013-11-26T09:58:33Z
dc.date.issued 2013-10
dc.description.abstract 2-Methoxyestradiol, a natural metabolite of estradiol, exerts antiproliferative and antitumour properties in vitro and in vivo. Because of its low oral bioavailability, several promising analogues of 2-methoxyestradiol have been developed. In this study, the in vitro influence of the compound, 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10)16-tetraene (C19), a non-commercially available 17-b-estradiol analogue, was tested on the breast adenocarcinoma MCF-7 cell line. The in vitro influence of 24 h exposure to 0.18 mM of C19 on MCF-7 cells was evaluated on cell morphology, cell cycle progression and possible induction of apoptosis and autophagy. Polarization-optical transmitted light differential interference contrast and fluorescence microscopy revealed the presence of cells blocked in metaphase, occurrence of apoptotic bodies and compromised cell density in C19-treated cells. Hallmarks of autophagy, namely an increase in the number of acidic vacuoles and lysosomes, were also observed in C19-treated samples. An increase in the number of cells present in the sub-G1 fraction, as well as a reduction in mitochondrial membrane potential was observed. No significant alterations in caspase 8 activity were observed. A twofold increase in aggresome formation was observed in C19-treated cells. C19 induced both apoptosis and autophagy in MCF-7 cells. en_US
dc.description.librarian hb2013 en_US
dc.description.librarian ay2013 en
dc.description.sponsorship The Medical Research Council of South Africa (AK076; AL343), the Cancer Association of South Africa (AK246), RESCOM, School of Medicine (Faculty of Health Sciences, University of Pretoria), the National Research Foundation of South Africa (AL239) and the Struwig-Germeshuysen Cancer Research Trust of South Africa (AN074). The Bioinformatics and Computational Biology Unit (University of Pretoria) contributed to the in silico-design of the compound. en_US
dc.description.uri http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1099-0844 en_US
dc.identifier.citation Nkandeu, DS, Mqoco, TV, Visagie, MH, Stander, BA, Wolmarans, E, Cronje, MJ & Joubert, AM 2013, 'In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-beta-estradiol analogue in breast adenocarcinoma cells', Cell Biochemistry and Function, vol. 31, no. 7, pp. 566-574. en_US
dc.identifier.issn 0263-6484 (print)
dc.identifier.issn 1099-0844 (online)
dc.identifier.other 10.1002/cbf.2937
dc.identifier.uri http://hdl.handle.net/2263/32614
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.rights © 2013 John Wiley & Sons, Ltd.This is the pre-peer reviewed version of the following article : In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-beta-estradiol analogue in breast adenocarcinoma cells in Cell Biochemistry and Function, vol. 31, no. 7, pp.566-574, 2013. doi : 10.1002/cbf.2937 which has been published in final form at : http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1099-0844 en_US
dc.subject 2-Methoxyestradiol (2ME2) en_US
dc.subject MCF-7 en_US
dc.subject Autophagy en_US
dc.subject Apoptosis en_US
dc.subject.lcsh Breast -- Cancer -- Research -- South Africa en
dc.title In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-beta-estradiol analogue in breast adenocarcinoma cells en_US
dc.type Preprint Article en_US


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