dc.contributor.author |
Joubert, Annie M.
|
|
dc.contributor.author |
Bianchi, Pepita
|
|
dc.contributor.author |
Maritz, Christine
|
|
dc.contributor.author |
Joubert, Fourie
|
|
dc.date.accessioned |
2007-07-20T11:48:13Z |
|
dc.date.available |
2007-07-20T11:48:13Z |
|
dc.date.issued |
2006-08 |
|
dc.description.abstract |
The effects of 20 μg/mL exogenous prostaglandin A2 (PGA2) were determined on Bax, Bcl-2 and proliferating cell nuclear antigen (PCNA) expression levels in MCF-7 cells. Flow cytometric analysis indicated a pronounced increase in the S phase and a decrease in the G1 phase, whereas a significant increase in the DNA content preceding the G0/G1 peak was also observed after 48 h of exposure to PGA2. Confirmation of apoptosis was determined after 12 h, 36 h and 48 h of PGA2 exposure employing the mitosensor reagent that detects potential changes in the mitochondrial membrane. Twenty-eight percent of PGA2-exposed cells were in apoptosis when compared to the 7.1% vehicle-treated cells after 48 h. PGA2 exposure led to statistically significant increase (1.25-fold) over vehicle-treated controls in Bax expression levels. Decreases in Bcl-2 (0.79-fold), as well as PCNA (0.69-fold) expression levels over vehicle-treated controls were observed. The Bax/Bcl-2 ratio for PGA2-exposed cells was 2.7. The present study suggests that an accumulation in the S phase, a decrease in expression levels of PCNA, as well as an altered ratio in favor of Bax, could lead to the induction of apoptosis in these cells. |
en |
dc.description.sponsorship |
This study was supoorted by the grants from the University of Pretoria, Cancer Association of South Africa, the Medical Research Council and THRIP. |
en |
dc.format.extent |
265659 bytes |
|
dc.format.mimetype |
application/pdf |
|
dc.identifier.citation |
Joubert, A, Bianchi, P, Maritz, C & Joubert, F 2006, 'Influence of prostaglandin A2 on Bax, Bcl-2 and PCNA expression in MCF-7 cells', Biomedical Research, vol. 27, no. 4, pp. 157-162. |
en |
dc.identifier.issn |
0388-6107 |
|
dc.identifier.issn |
1880-313X |
|
dc.identifier.other |
10.2220/biomedres.27.157 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/3086 |
|
dc.language.iso |
en |
en |
dc.publisher |
Biomedical Research Press |
en |
dc.rights |
Biomedical Research Press |
en |
dc.subject |
Exogenous prostaglandin |
en |
dc.subject |
Flow cytometric analysis |
en |
dc.subject.lcsh |
Prostaglandins |
|
dc.subject.lcsh |
Flow cytometry--Diagnostic use |
|
dc.subject.lcsh |
Apoptosis |
|
dc.subject.lcsh |
Mitochondrial membranes |
|
dc.title |
Influence of prostaglandin A2 on Bax, Bcl-2 and PCNA expression in MCF-7 cells |
en |
dc.type |
Article |
en |