RESTseq – efficient benchtop population genomics with RESTriction fragment SEQuencing

Stolle, Eckart; Moritz, Robin F.A.

UPSpace Home
→
Natural and Agricultural Sciences
→
Zoology and Entomology
→
Research Articles (Zoology and Entomology)
→
View Item

dc.contributor.author	Stolle, Eckart
dc.contributor.author	Moritz, Robin F.A.
dc.contributor.editor	Wade, Claire
dc.date.accessioned	2013-07-12T07:23:14Z
dc.date.available	2013-07-12T07:23:14Z
dc.date.issued	2013-05-17
dc.description.abstract	We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome sequencers. Easy adjustments make it suitable for a wide range of studies requiring SNP detection or SNP genotyping from fine-scale linkage mapping to population genomics and population genetics also in non-model organisms. We demonstrate the validity of our approach by comparing two honeybee and several stingless bee samples.	en
dc.description.librarian	am2013	en
dc.description.librarian	ab2013
dc.description.sponsorship	European Union’s Seventh Framework Programme (grant agreement BEE DOC FP7-KBBE-2009-3 244956 CP-FP), Bundesministerium fu¨ r Erna¨hrung, Landwirtschaft und Verbraucherschutz (project FITBEE).	en
dc.description.uri	http://www.plosone.org	en
dc.identifier.citation	Stolle E, Moritz RFA (2013) RESTseq – Efficient Benchtop Population Genomics with RESTriction Fragment SEQuencing. PLoS ONE 8(5): e63960. DOI: 10.1371/journal.pone.0063960	en
dc.identifier.issn	1932-6203
dc.identifier.other	10.1371/journal.pone.0063960
dc.identifier.uri	http://hdl.handle.net/2263/21910
dc.language.iso	en	en
dc.publisher	Public Library of Science	en
dc.rights	© 2013 Stolle, Moritz. This is an open-access article distributed under the terms of the Creative Commons Attribution License	en
dc.subject	RESTseq	en
dc.subject	SNP detection	en
dc.subject	SNP genotyping	en
dc.subject	Next generation sequencing (NGS)	en
dc.subject.lcsh	Metagenomics	en
dc.subject.lcsh	DNA	en
dc.title	RESTseq – efficient benchtop population genomics with RESTriction fragment SEQuencing	en
dc.type	Article	en