dc.contributor.author |
Butler, Catherine M.
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dc.contributor.author |
Sloet van Oldruitenborgh-Oosterbaan, Marianne M.
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dc.contributor.author |
Stout, T.A.E. (Tom)
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dc.contributor.author |
Van der Kolk, Johannes H.
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dc.contributor.author |
Van den Wollenberg, Linda
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dc.contributor.author |
Nielen, Mirjam
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dc.contributor.author |
Jongejan, Frans
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dc.contributor.author |
Werners, Arno H.
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dc.contributor.author |
Houwers, Dirk J.
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dc.date.accessioned |
2012-10-05T07:11:38Z |
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dc.date.available |
2012-10-05T07:11:38Z |
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dc.date.issued |
2012-08 |
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dc.description.abstract |
Equine piroplasmosis (EP) has not been considered indigenous in The Netherlands. However, following
the detection of an apparently indigenous subclinical Babesia caballi infection in a horse on Schouwen-
Duiveland (an island in the Zeeland Province), a survey was undertaken between May and September
2010 to assess the prevalence of the causative agents of EP in the South-West of The Netherlands. Blood
samples from 300 randomly selected horses were tested for specific antibodies against Theileria equi and
B. caballi using an indirect fluorescence antibody test (IFAT), and for parasite DNA using a specific polymerase
chain reaction combined with reverse line blotting (PCR-RLB).
Twelve of the horses (4%) were seropositive for EP. Of these, nine (75%) were positive (titreP1:160)
for B. caballi alone and three (25%) were also positive for T. equi. PCR-RLB detected T. equi DNA in five
horses (1.6%), two of which were seronegative. Four (1.3%) of the positive horses (three positive for T. equi
and one for both B. caballi and T. equi) were considered truly indigenous.
During the study, two indigenous ponies from a farm situated outside the sampling area were diagnosed
with acute clinical piroplasmosis characterized by severe anaemia and pyrexia. Blood smears
showed T. equi – like inclusions in red blood cells, and T. equi infection was confirmed in both ponies
by PCR-RLB. The initial subclinical B. caballi infection, the survey results and the two acute clinical EP
cases confirmed the autochthonous transmission of B. caballi and T. equi infections in The Netherlands. |
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dc.description.librarian |
ab2012 |
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dc.description.sponsorship |
This research was partly funded by the Dutch Ministry of Agriculture. |
en |
dc.description.uri |
http://www.elsevier.com/locate/tvjl |
en |
dc.identifier.citation |
Catherine M. Butler, Marianne M. Sloet van Oldruitenborgh-Oosterbaan, Tom A.E. Stout, Johannus H. van der Kolk, Linda van den Wollenberg, Mirjam Nielen, Frans, Jongejan, Arno H. Werners & Dirk J. Houwers, Prevalence of the causative agents of equine piroplasmosis in the South West of the Netherlands and the identification of two autochthonous clinical Theileria equi infections, The Veterinary Journal, vol. 193, no. 2, pp. 381-385 (2012), doi: 10.1016/j.tvjl.2011.12.014. |
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dc.identifier.issn |
1090-0233 (print) |
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dc.identifier.other |
10.1016/j.tvjl.2011.12.014 |
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dc.identifier.uri |
http://hdl.handle.net/2263/19996 |
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dc.language.iso |
en |
en |
dc.publisher |
Elsevier |
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dc.relation.requires |
Adobe Acrobat Reader |
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dc.rights |
© 2011 Elsevier. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in The Veterinary Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in The Veterinary Journal, vol 193, issue 2, August 2012, doi: 10.1016/j/tvjl.2011.12.014. |
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dc.subject |
Piroplasmosis |
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dc.subject |
Babesia caballi |
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dc.subject |
Theileria equi |
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dc.subject |
PCR-RLB |
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dc.subject.lcsh |
Babesiosis in horses |
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dc.subject.lcsh |
Horses -- Diseases |
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dc.subject.lcsh |
Theileria |
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dc.subject.lcsh |
Babesia |
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dc.subject.lcsh |
Polymerase chain reaction |
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dc.title |
Prevalence of the causative agents of equine piroplasmosis in the South West of the Netherlands and the identification of two autochthonous clinical Theileria equi infections |
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dc.type |
Postprint Article |
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