dc.contributor.author |
Ferrer, Manuel
|
|
dc.contributor.author |
Ghazi, Azam
|
|
dc.contributor.author |
Beloqui, Ana
|
|
dc.contributor.author |
Vieites, Jose Marıa
|
|
dc.contributor.author |
Lopez-Cortes, Nieves
|
|
dc.contributor.author |
Marın-Navarro, Julia
|
|
dc.contributor.author |
Nechitaylo, Taras Y.
|
|
dc.contributor.author |
Guazzaroni, Marıa-Eugenia
|
|
dc.contributor.author |
Polaina, Julio
|
|
dc.contributor.author |
Waliczek, Agnes
|
|
dc.contributor.author |
Chernikova, Tatyana N.
|
|
dc.contributor.author |
Reva, Oleg N.
|
|
dc.contributor.author |
Golyshina, Olga V.
|
|
dc.contributor.author |
Golyshin, Peter N.
|
|
dc.date.accessioned |
2012-08-30T11:59:43Z |
|
dc.date.available |
2012-08-30T11:59:43Z |
|
dc.date.issued |
2012-06-25 |
|
dc.description.abstract |
Microbial communities from cow rumen are known for their ability to degrade diverse plant polymers at high rates. In this
work, we identified 15 hydrolases through an activity-centred metagenome analysis of a fibre-adherent microbial
community from dairy cow rumen. Among them, 7 glycosyl hydrolases (GHs) and 1 feruloyl esterase were successfully
cloned, expressed, purified and characterised. The most striking result was a protein of GH family 43 (GHF43), hereinafter
designated as R_09-02, which had characteristics very distinct from the other proteins in this family with mono-functional bxylosidase,
a-xylanase, a-L-arabinase and a-L-arabinofuranosidase activities. R_09-02 is the first multifunctional enzyme to
exhibit b-1,4 xylosidase, a-1,5 arabinofur(pyr)anosidase, b-1,4 lactase, a-1,6 raffinase, a-1,6 stachyase, b-galactosidase and a-
1,4 glucosidase activities. The R_09-02 protein appears to originate from the chromosome of a member of Clostridia, a class
of phylum Firmicutes, members of which are highly abundant in ruminal environment. The evolution of R_09-02 is
suggested to be driven from the xylose- and arabinose-specific activities, typical for GHF43 members, toward a broader
specificity to the glucose- and galactose-containing components of lignocellulose. The apparent capability of enzymes from
the GHF43 family to utilise xylose-, arabinose-, glucose- and galactose-containing oligosaccharides has thus far been
neglected by, or could not be predicted from, genome and metagenome sequencing data analyses. Taking into account the
abundance of GHF43-encoding gene sequences in the rumen (up to 7% of all GH-genes) and the multifunctional phenotype
herein described, our findings suggest that the ecological role of this GH family in the digestion of ligno-cellulosic matter
should be significantly reconsidered. |
en_US |
dc.description.sponsorship |
The Spanish CDTI (research projects CENIT 2007-1031 and Cenit BioSos, I+DEA), the Ministry of Economy and Competitiveness «Fondo de inversion local para el empleo-Gobierno de Espana». AB thanks the
Spanish MEC for a FPU fellowship. M-EG thanks the CSIC for a JAE-Doc fellowship. TYN, PNG, and OVG acknowledge the support of grant 0313751K from the
Federal Ministry for Science and Education (BMBF) within the GenoMikPlus initiative. |
en_US |
dc.description.uri |
http://www.plosone.org |
en_US |
dc.identifier.citation |
Ferrer M, Ghazi A, Beloqui A, Vieites JM, Lopez-Cortes N, et al. (2012) Functional Metagenomics Unveils a Multifunctional Glycosyl Hydrolase from the Family 43 Catalysing the Breakdown of Plant Polymers in the Calf Rumen. PLoS ONE 7(6): e38134. DOI: 10.1371/journal.pone.0038134. |
en_US |
dc.identifier.issn |
1932-6203 |
|
dc.identifier.other |
10.1371/journal.pone.0038134 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/19683 |
|
dc.language.iso |
en |
en_US |
dc.rights |
© 2012 Ghazi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License. |
en_US |
dc.subject |
Calf rumen |
en_US |
dc.subject |
Breakdown of plant polymers |
en_US |
dc.title |
Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumen |
en_US |
dc.type |
Article |
en_US |