Herr, S.Brett, O.L.Bigalke, R.D.2015-03-202015-03-2020141986Gummow, B, Herr, S & Brett, OL 1986, 'A short, reliable, highly reproducible complement fixation test for the serological diagnosis of contagious equine metritis’, Onderstepoort Journal of Veterinary Research, vol. 53, no. 4, pp. 241-243.0330-24657003908833N-9314-2014http://hdl.handle.net/2263/44095The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A complement fixation test, using round-bottomed microtitration plates and an 8 channel microdiluter, based on that used for brucellosis by Herr, Huchzermeyer, Te Brugge, Williamson, Roos & Schiele, 1985, has been developed for use on the sera of horses to detect antibodies to the contagious equine metritis organism. The results with 2 known positive sera tested 116 times in 27 separate tests were reproducible for the most part within a two fold range. They seldom exceeded these limits and never exceeded a fourfold range. The test itself is capable of being carried out within 90 min. The test was slightly more sensitive when sera were inactivated in a hot air oven for 50 min at 58 °C, as compared to inactivation at 62 °C in a water-bath for 50 min. There were no false negative or false positive reactions and no anticomplementary activity in the sera tested.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle