Viljoen, G.J.Bezuidenhout, J. DürrOberem, P.T.Vermeulen, N.M.J.Van Wyngaardt, WouterBigalke, R.D.2014-12-052014-12-0520141986Neitz, AWH, Viljoen, GJ, Bezuidenhout, JD, Oberem, PT, Van Wyngaardt, W & Vermeulen, NMJ 1986, 'The detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum by an enzyme-linked immunosorbent assay’, Onderstepoort Journal of Veterinary Research, vol. 53, no. 1, pp. 39-41.0330-2465http://hdl.handle.net/2263/42800The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A sensitive and reliable enzyme-linked immunosorbent assay for the detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum nymphae is described. For the screening of antibodies, C. ruminantium from A. hebraeum nymphae, partially purified by wheat-germ lectin affinity chromatography, was used as antigen. To screen nymph populations, sera from either Ball 3 strain-infected sheep or Kumm-strain infected mice were used. By using appropriate controls the assays were rendered specific with respect to C. ruminantium.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle