Asri, BtissamTahir, DjamelEvans, AlecMeyer, LeonRhalem, AbdelkbirBouslikhane, MohammedUeti, MassaroMadder, Maxime2024-09-062024-09-062023-03-24Asri, B.; Tahir, D.; Evans, A.; Meyer, L.N.; Rhalem, A.; Bouslikhane, M.; Ueti, M.; Madder, M. Assessment of an In Vitro Tick Feeding System for the Successful Feeding of Adult Rhipicephalus appendiculatus Ticks. Parasitologia 2023, 3, 101–108. https://DOI.org/10.3390/parasitologia3020012.2673-677210.3390/parasitologia3020012http://hdl.handle.net/2263/98067DATA AVAILABILITY STATEMENT : The data presented in this study are available in the article.This study assessed the efficiency of a new in vitro tick feeding system for the adult Rhipicephalus appendiculatus tick and compared the impact of different blood anticoagulating factors on their feeding process. A total of 10 feeders were each seeded with 30 or 60 R. appendiculatus adults. Bovine blood was added into each unit and changed every 12 h for 4 to 10 days during which tick attachment and engorgement was assessed. The tick attachment observed 4 days after feeding was 80.0% (48/60), 75.8% (182/240), and 70.8% (170/240) for lithium heparin, citrate phosphate dextrose, and defibrinated blood, respectively, with no significant difference (p > 0.05) between the anticoagulants used. However, the ticks fed on heparinized and defibrinated blood reached repletion status. The in vitro tick feeding system was successfully used to feed adult R. appendiculatus ticks until repletion. This system could be used to facilitate studies on tick-pathogen interactions, such as those involved in the East Coast fever disease.en© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.Rhipicephalus appendiculatusTheileria parvaIn vitro tick feedingEast Coast feverTicksSDG-03: Good health and well-beingArticle