Titoy, G.A.P.Schwan, Ernst VolkerVerwoerd, Daniel Wynand2013-09-032013-09-0320131995Titoy, GAP & Schwan, EV 1995, 'Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 1, pp. 63-64.0330-2465http://hdl.handle.net/2263/22262The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A technique for the cryopreservation of third stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed third-stage larvae in 80% (v/v) ethylene glycol as a cryoprotectant for 30 s at 0°C, prior to transfer into liquid nitrogen. The survival rate, as assessed by motility, was 37,6% after 30 d cryopreservation. A sheep infected percutaneously with 700 live cryopreserved third-stage larvae, harboured 41 adult worms (infectivity rate: 5,8%) when necropsied 78 d later.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineCryopreservationSheathed third-stage larvaeGaigeria pachyscelisSandveld hookwormVeterinary medicine -- South AfricaArticle