Lukusa, KambuluKabuba, John2022-02-042022-02-042021-07-20Lukusa, K. & Kabuba, J. Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks. Asian Pacific Journal of Reproduction 2021; 10(4): 187-194.2305-0500 (print)2305-0519 (online)10.4103/2305-0500.321126http://hdl.handle.net/2263/83632OBJECTIVE : To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks. METHODS : Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10- day intervals for three months. Sperm kinematic parameters, morphological parameters, mitochondrial membrane potential, plasma membrane functionality, and sperm DNA integrity were evaluated weekly pre and post-cryopreservation. RESULTS : The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method (P<0.05). Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation (P<0.05). Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the seleniumsupplemented bucks compared to the electro-ejaculation and the control groups (P<0.05). The sperm kinematic parameters such as curvilinear velocity, average path velocity, and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05). The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups (P<0.05). In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups, respectively (P<0.05). CONCLUSIONS : Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.en© 2021 Asian Pacific Journal of Reproduction. This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-Share Alike 4.0 License.Post thawCryopreservationSaanen buckSelenium supplementationSemen collection methodSperm kinematic parametersSperm motilitySperm qualitySaanen bucksArticle