Chisi, Songelwayo L.Marageni, YoandaNaidoo, PrebashniZulu, GloriaAkol, George W.Van Heerden, Henriette2018-01-112018-01-112017-02-28Chisi, S.L., Marageni, Y., Naidoo, P., Zulu, G., Akol, G.W. & Van Heerden, H., 2017, ‘An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa’, Journal of the South African Veterinary Association 88(0), a1381. https://DOI.org/ 10.4102/jsava.v88i0.1381.1019-9128 (print)2224-9435 (online)10.4102/jsava.v88i0.1381http://hdl.handle.net/2263/63477The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples. The diagnostic specificity (DSp) of the tests mentioned above was determined using samples from known negative herds. There was no statistically significant difference between the tests in their ability to diagnose brucellosis. The RBT and iELISA had the highest DSe of 95.8%, whereas RBT and CFT had the highest DSp of 100%. In South African laboratories, the RBT and CFT serological tests are used, because of the cost efficacy of CFT when compared to the less labour intensive but more expensive iELISA.en© 2017. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.South African laboratoriesDiagnostic sensitivity (DSe)Rose Bengal test (RBT)Indirect ELISA (iELISA)Competitive enzyme-linked immunosorbent assay (cELISA)Serum agglutination test (SAT)Complement fixation test (CFT)Serological testsBovine brucellosisCattleKwaZulu-NatalArticle