Mensah, Deborah L. NarhWingfield, Brenda D.Coetzee, Martin Petrus Albertus2024-03-132024-03-132023-09-08Mensah, D.L.N., Wingfield, B.D., Coetzee, M.P.A. 2023, 'A practical approach to genome assembly and annotation of Basidiomycota using the example of Armillaria', BioTechniques, vol. 75, no. 3, pp. 115-128. DOI : 10.2144/btn-2023-0023.0736-6205 (print)1940-9818 (online)10.2144/btn-2023-0023http://hdl.handle.net/2263/95189SUPPLEMENTARY DATA : To view the supplementary data that accompany this paper please visit the journal website at: www.future-science.com/doi/suppl/10. 2144/btn-2023-0023Technological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects are evaluated in this review. Infographic guidelines and a database of resources to facilitate future Armillaria genome projects were developed. Knowledge gained from genomic studies of Armillaria species is summarized and prospects for further research are provided. This guide can be applied to other diploid and dikaryotic fungal genomics.en© 2023 University of Pretoria. This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License.BioinformaticsDNA extractionEvolutionGenomicsMetabolomicsNext-generation sequencingPhylogenomicsPhytopathogensProteomicsTranscriptomicsDeoxyribonucleic acid (DNA)Article