Atkinson, BroganGoddard, AmeliaEngelbrecht, MoniquePretorius, S.Pazzi, Paolo2023-08-242023-08-242022Atkinson, B.K., Goddard, A., Engelbrecht, M. et al. 2022, 'Circulating markers of endothelial activation in canine parvoviral enteritis', Journal of the South African Veterinary Association, vol. 93, no. 1, pp. 1-7. https://DOI.org/10.36303/JSAVA.2022.93.1.496.1019-9128 (print)2224-9435 (online)10.36303/JSAVA.2022.93.1.496http://hdl.handle.net/2263/92018The data that support the findings of this study are available from the corresponding author, B.K. Atkinson, upon reasonable request.BACKGROUND : Canine parvovirus (CPV) is a common cause of enteritis, immune suppression and systemic inflammation in young dogs. Endothelial markers, such as intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1), and molecules that upregulate their expression, such as high mobility group box 1 protein (HMGB-1), provide insight into the state of the endothelium during inflammation. OBJECTIVES : This study aimed to determine if circulating concentrations of ICAM-1, VCAM-1 and HMGB-1 were altered in CPV enteritis compared to healthy controls, and whether a correlation existed between these molecules and the degree of inflammation METHODS : Thirty dogs with naturally occurring CPV enteritis and ten control dogs were included. Physical examinations, complete blood count and C-reactive protein (CRP) measurements were performed on all dogs at presentation. The concentrations of ICAM-1, VCAM-1 and HMGB-1 were measured using commercially available canine-specific enzyme-linked immunosorbent assay (ELISA) kits. RESULTS : In dogs with CPV enteritis, ICAM-1 concentrations were significantly lower (median: 5.9 [IQR: 4.3-8.3]) and CRP higher (134 [IQR: 85-195]) compared to controls (8.0 [IQR: 6.9-10.3], p = 0.008; 1 [IQR: 0-7], p < 0.001). No significant difference was found for VCAM-1 and HMGB-1. A strong correlation was identified between VCAM-1 and segmented neutrophil count (r = 0.612, p < 0.001). CONCLUSION : Despite the presence of systemic inflammation in CPV enteritis, evidenced by high CRP concentrations, our results suggest circulating concentrations of ICAM-1, VCAM-1 and HMGB-1 failed to show an increase. Endothelial activation with subsequent leukocyte adhesion and transmigration through the endothelium may be affected in CPV enteritis and these findings require further investigation.en© 2022 The Author(s). Open Access article distributed under the terms of the Creative Commons License [CC BY-NC 3.0].LeukocytesCanine parvovirus (CPV)Intercellular adhesion molecule 1 (ICAM-1)Vascular cell adhesion molecule 1 (VCAM-1)High mobility group box 1 protein (HMGB-1)Dogs (Canis familiaris)SDG-03: Good health and well-beingArticle