Haig, D.A.Du Toit, P.J.2017-04-062017-04-0620171948Haig, DA 1948, 'Preliminary note on the cultivation of Green's distemperoid virus in fertile hen eggs’, Onderstepoort Journal of Veterinary Science and Animal Industry, vol. 23, nos. 1 & 2, pp. 149-155.0330-2465http://hdl.handle.net/2263/59693The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Green's distemper vaccine virus has been propagated 30 generations in developing hen eggs. Injections were made onto the chorio-allantoic membranes of eggs that had received a preliminary incubation of 8 days. Re-incubation was done at 35°C., and passage of the membranes was made at approximately 4 day intervals. The egg-adapted strain produced markedly oedematous changes with some necrosis in the membranes, but only occasionally killed the embryo. The titre of infected chorio-allantoic membranes, measured by ferret injection was between 10⁻³ and 10⁻⁵. That of the embryos and extra-enbryonic fluids of the same eggs was 10⁻³. The reactions produced by the egg-adapted strain when injected into ferrets were similar to those produced by the parent strain. Five c.c. of anti-distemper serum neutralized the egg-cultured virus. Thirteen dogs were inoculated with suspensions of chorio-allantoic membranes. In nine there was no reaction. In one there was a slight reaction, but in three the reactions were very severe and one died after showing typical distemper symptoms. The egg-adapted virus did not agglutinate chicken or guinea pig red cells and failed to infect mice when instilled intra-nasally.en© 1948 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © 2017 University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle