Wu, H.C. (How-Chiun)Du Toit, Elsa SophiaReinhardt, Carl Frederick (Charlie)2008-05-272008-05-272007-04Wu, HC, Du Toit, ES & Reinhardt, CF 2007, ‘Micrografting of Protea cynaroides’, Plant Cell, Tissue and Organ Culture, vol. 89, no. 1, pp. 23-28. [www.springerlink.com]0167-68571573-504410.1007/s11240-007-9208-5http://hdl.handle.net/2263/5581The inability to induce rooting of in vitro-established Protea cynaroides microshoots has prevented the production of complete plantlets. A successful shoot-tip micrografting technique was developed using in vitro-germinated P. cynaroides seedlings as rootstocks and axenic microshoots established from pot plants as microscions. Thirty-day old seedlings, germinated on growth-regulator-free, half-strength Murashige and Skoog medium, were decapitated and a vertical incision made from the top end. The bottom ends of microshoots established on modified Murashige and Skoog medium were cut into a wedge (‘V’) shape, and placed into the incision. The micrografted explants were cultured in a growth chamber with the temperature adjusted to 25 ± 2°C, with a 12-h photoperiod. Best results were obtained by placing the microscions directly onto the rootstock without any pre-treatments. Dipping the explants in anti-oxidant solution or placing a layer of medium around the graft area led to the blackening of the microscion. Abbreviations EDTA Ethylenediaminetetraacetate - BAP 6-Benzylaminopurine - GA3 Gibberellic acid - PAR Photosynthetic active radiation162968 bytesapplication/pdfenSpringer. The original publication is available at www.springerlink.comGraftingIn vitroKing ProteaMicropropagationProteaceaeProteaceae -- MicropropagationPostprint Article