Heartwater : Past, Present and Future. Workshop (1986 : Kruger National Park, South Africa)Du Plessis, J.L.Malan, LetitiaBigalke, R.D.2014-11-192014-11-1920141987Du Plessis, JL & Malan, L 1987, 'The application of the indirect fluorescent antibody test in research on heartwater’, Onderstepoort Journal of Veterinary Research, vol. 54, no. 3, pp. 319-325.0330-2465http://hdl.handle.net/2263/42666The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The preparation of the antigen, details of the reagents, the titration of the antispecies conjugates and the execution of the indirect fluorescent antibody test are described. The sensitivity and specificity of the test and its applicability to the detection of antibodies to Cowdria ruminantium are recorded. The test is both highly specific and sensitive and can be applied to a wide range of studies on heartwater, including epidemiology, determination of the C. ruminantium infection rate of Amblyomma ticks and the evaluation of immunization against heartwater. The test can also be used to detect antibodies to the heartwater agent in the sera of game.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaHeartwater -- South AfricaArticle