Chandler, Jennifer C.Jafree, Daniyal J.Malik, SaifPomeranz, GideonBall, MaryKolatsi-Joannou, MariaPiapi, AliceMason, William J.Benest, Andrew V.Bates, David O.Letunovska, AleksandraAl-Saadi, ReemRabant, MarionBoyer, OliviaPritchard-Jones, KathyWinyard, Paul J.Mason, Andrew S.Woolf, Adrian S.Waters, Aoife M.Long, David A.2024-10-312024-10-312024-10Chandler J.C., Jafree D.J., Malik S. et al. 2024, 'Single-cell transcriptomics identifies aberrant glomerular angiogenic signalling in the early stages of WT1 kidney disease', Journal of Pathology, vol. 264, no. 2, pp. 212-227, doi : 10.1002/path.6339.0022-3417 (print)1096-9896 (online)10.1002/path.6339http://hdl.handle.net/2263/98857DATA AVAILABILITY STATEMENT : Primary sequencing data reported in this paper are available on NCBI Sequence Read Archive (SRA PRJNA928337). Processed data for scRNA-seq experiments are available on Zenodo at DOI: 10.5281/zenodo.7565867. The codes generated during this study are available at Github repository https://github.com/daniyal-jafree1995/collaborations. All other unique reagents used in this study are available from the corresponding author on reasonable request.SUPPLEMENTARY MATERIALS AND METHODS : FIGURE S1. Supporting data for single-cell RNA seq analyses. FIGURE S2. Characterisation of primary cell lines. FIGURE S3. Isolation of podocytes from scRNA-seq data from additional murine models of glomerular disease for cross-disease comparison. FIGURE S4. Dysregulated genes shown by individual sample for each human glomerular pathology (source Nephroseq). TABLE S1. Differentially expressed genes in glomerular cells in Wt1R394W/+ mice. TABLE S2. Podocyte differentially expressed genes that are conserved across murine glomerular scRNA-seq datasets or unique to Wt1R394W/+. TABLE S3. Podocyte differentially expressed genes classified in ‘GO:0002376 – immune system process’ across murine glomerular scRNA-seq datasets.Please read abstract in the article.en© 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. This is an open access article under the terms of the Creative Commons Attribution License.Vascular endothelial growth factor-AWT1 glomerulopathyWilms tumour 1 (WT1)Single-cell transcriptomicsPodocyteNeuropilin-1Glomerular endotheliumChildhood glomerular diseaseAdrenomedullinSDG-03: Good health and well-beingArticle