Verwoerd, Daniel Wynand2013-08-292013-08-2920121996Van Niekerk, CJ & Zweygarth, E 1996, 'In vitro cultivation of Babesia occultans’. Onderstepoort Journal of Veterinary Research, vol. 63, no. 3, pp. 259-261.0330-2465http://hdl.handle.net/2263/22096The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Babesia occultans, the causative agent of a benign form of cattle babesiosis in South Africa, was continuously cultivated in microaerophilous stationary-phase culture. A modified medium, 199, supplemented with either 40% (v/v) bovine or 40% (v/v) horse serum, was used. Cultures were initiated in a humidified atmosphere containing 2% 0₂, 5% C0₂, and 93% N₂. The highest percentage of parasitized erythrocytes (PPE) reached 4,5% in horse-serum- and 2,4% in bovine-serum-supplemented medium. Parasite suspensions were cryopreserved and successfully resuscitated.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineIn vitro cultivationBabesia occultansBovine babesiosisSouth AfricaVeterinary medicine -- South AfricaBabesiosis in cattle -- South AfricaArticle