Shipham, S.O.De la Rey, MarleneBigalke, R.D.Morren, A.J.Verster, Anna J.M.Verwoerd, Daniel WynandWalker, Jane B.Cameron, Colin McKenzieGilchrist, Frances M.C.Steyn, P.J.J.2016-07-062016-07-0620161979Shipham, SO, De la Rey, M 1979, 'Genetic and physiological characterization of temperature-sensitive mutants of bluetongue virus’, Onderstepoort Journal of Veterinary Research, vol. 46, no. 2, pp. 87-94.0330-2465http://hdl.handle.net/2263/53755This article has been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-Format.Complementation studies were carried out, using temperature-sensitive (t-s) mutants of bluetongue virus (BTV). The results proved to be inconclusive as only low indices of complementation were obtained. No discrepancy was found between the previous classification of these mutants in 6 recombination classes and the complementation data recorded. In general, the t-s mutants require a latent growth period of 16-20 h at 28 °C and maximum titres can be demonstrated 40-48 h post-infection. One mutant, (F211), however, consistently had a growth lag phase of 32 h. Mutants of the 6 recombination groups were further classified into 2 groups by temperature-shift studies. One class of mutants expressed their t-s lesion prior to 24 h and the other class only after 24 h post-infection. Mutant F73 was found to be defective in its ability to synthesize ssRNA at a late stage in the replication cycle at the non-permissive temperature.en©1979 ARC - Ondersterpoort and Faculty of Veterinary Science, University of Pretoria (original). ©2016 University of Pretoria Department of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle