Anderson, L.A.P.Schultz, R.A. (Rowena Anitra)Kellerman, T. StephanusKotze, S.M.Erasmus, G.L.Labuschagne, LeonieBigalke, R.D.2015-03-312015-03-3120141985Anderson, LAP, Schultz, RA, Kellerman, TS, Kotze, SM, Prozesky, L, Erasmus, GL & Labuschagne, L 1985, 'Isolation and characterization of and some observations on poisoning by bufadienolides from Cotyledon orbiculata L. var. orbiculata’, Onderstepoort Journal of Veterinary Research, vol. 52, no. 1, pp. 21-24.0330-2465http://hdl.handle.net/2263/44225The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The approximate LD₅₀ and cumulative effect of 4 bufadienolides, isolated from Cotyledon orbiculata, were determined in guinea-pigs. Two of the bufadienolides proved to be mildly cumulative. One of the mildly cumulative bufadienolides induced subacute intoxication with some signs of krimpsiekte when injected intravenously into a sheep over 13 days. In the sheep, the paralysis was accompanied by signs of malaise, such as electrocardiographic changes and ruminal stasis.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicineArticle