Shipham, S.O.De la Rey, M.Bigalke, R.D.Cameron, Colin McKenzieGilchrist, Frances M.C.Morren, A.J.Verster, Anna J.M.Verwoerd, Daniel WynandWalker, Jane B.De Kock, V.E.2016-07-062016-07-0620161976Shipham, SO & De la Rey, M 1976, 'The isolation and preliminary genetic classification of temperature-sensitive mutants of bluetongue virus’, Onderstepoort Journal of Veterinary Research, vol. 43, no. 4, pp. 189-192.0330-2465http://hdl.handle.net/2263/53663The articles have been scanned in colour with a HP scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to final presentyation PDF-Format.Temperature-sensitive mutants of bluetongue virus were isolated and classified in 6 genetic recombination groups. The frequency of recombination varied both within and between groups. The 4 mutagens used viz. nitrous acid, N-methyl-N-nitroso-N-nitroguanidine, proflavine and 5-fluorouracil were found to differ in their efficacy. The period of incubation required for maximum recombination was 48 h at 28 °C.en©1976 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©2016 University of Pretoria. Department of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle