Snyman, JumariSnyman, Louwrens PieterLabuschagne, KarienVenter, Gert JohannesVenter, Marietjie2022-06-212022-06-212021-07Snyman, J., Snyman, L.P., Labuschagne, K. et al. 2021, 'The utilisation of CytB and COI barcodes for the identification of bloodmeals and Culicoides species (Diptera: Ceratopogonidae) reveals a variety of novel wildlife hosts in South Africa', Acta Tropica, vol. 219, art. 105913, pp. 1-8, doi : 10.1016/j.actatropica.2021.105913.0001-706X (print)1873-6254 (online)10.1016/j.actatropica.2021.105913https://repository.up.ac.za/handle/2263/85886Biting midges in the genus Culicoides (Diptera; Ceratopogonidae) are vectors of pathogens that can cause diseases of major economic importance in humans and animals. Identifying host ranges of these biting midges might aid in understanding the complex epidemiology of such diseases, often involving reservoir hosts and multiple species. In this study, we aim to identify bloodmeal origin from engorged female Culicoides biting midges. All bloodfed females were opportunistically collected as part of an ongoing surveillance programme using Onderstepoort light traps in two provinces in South Africa. DNA of individuals was extracted and subjected to PCR targeting the cytochrome B (CytB) gene region of mammals and avians as well as cytochrome oxidase I (COI) for species identification. In total, 21 new reference barcodes were generated for C. bedfordi, C imicola, C. leucosticus, C. magnus, and C. pycnostictus. Seventy-four blood meals were identified, originating from 12 mammal and three avian species. COI sequence data performed well for species delimitation and 54 Culicoides specimens were identified with C. imicola the predominant species identified (41.8%). Generally, Culicoides species feed on a variety of hosts and host availability might be an important factor when selecting a host. Culicoides species thus appear to be opportunistic feeders rather than specialists. This implicates Culicoides as transfer vectors and demonstrates possible transmission routes of arboviruses and other pathogens from wildlife onwards to domestic animals and humans.en© 2021 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Acta Tropica. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Acta Tropica, vol. 219, art. 105913, pp. 1-8, 2021. doi : 10.1016/j.actatropica.2021.105913.VectorHaematophagyArbovirusReservoir hostDNA barcodeHost preferenceZoonosisPostprint Article