Aranda-Diaz, AndresVickers, Eric NeubauerMurie, KathrynPalmer, BrianHathaway, NicholasGerlovina, InnaBoene, SimoneGarcia-Ulloa, ManuelCistero, PauKatairo, ThomasSemakuba, Francis DdumbaNsengimaana, BienvenuGwarinda, HazelGarcia-Fernandez, CarlaLouie, WilliamEsayas, EndashawDa Silva, ClementeDatta, DebayanKiyaga, ShahiidWiringilimaana, InnocentFeleke, Sindew MekashaBennett, AdamSmith, Jennifer L.Gadisa, EndalamawParr, Jonathan B.Conrad, Melissa D.Raman, JaishreeTukwasibwe, StephenSsewanyana, IsaacRovira-Vallbona, EduardTato, Cristina M.Briggs, JessicaMayor, AlfredoGreenhouse, Bryan2025-09-122025-09-122025-03Aranda-Díaz, A., Neubauer Vickers, E., Murie, K. et al. Sensitive and modular amplicon sequencing of Plasmodium falciparum diversity and resistance for research and public health. Scientific Reports 15, 10737 (2025). https://doi.org/10.1038/s41598-025-94716-5.2045-2322 (online)10.1038/s41598-025-94716-5http://hdl.handle.net/2263/104307DATA AVAILABILITY: All data are available in the Sequencing Read Archive, accession code PRJNA1180199. Code is available in GitHub (https://github.com/EPPIcenter/mad4hatter and https://github.com/andres-ad/madh_utilities).Please read abstract in the article.en© The Author(s) 2025. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.MalariaPlasmodium falciparumTargeted amplicon sequencingMicrohaplotypeAntimalarial resistancehrp2 deletionCspIdentity-by-descent (IBD)Complexity of infectionWhole-genome sequencing (WGS)Article