Van Dijk, A.A.Bigalke, R.D.Cameron, Colin McKenzieGilchrist, Frances M.C.Jordaan, E.Morren, A.J.Verster, Anna J.M.Verwoerd, Daniel WynandWalker, Jane B.Steyn, P.J.J.2016-03-022016-03-0220151982Van Dijk, AA & Huismans, H 1982, 'The effect of temperature on the in vitro transcriptase reaction of bluetongue virus, epizootic haemorrhagic disease virus and African horsesickness virus’, Onderstepoort Journal of Veterinary Research, vol. 49, no. 4, pp. 227-232.0330-2465http://hdl.handle.net/2263/51647The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Virions of bluetongue virus (BTV), epizootic haemorrhagic disease virus (EHDV) and African horsesickness virus (AHSV) can be converted to core particles by treatment with chymotrypsin and magnesium. The conversion is characterized by the removal of the 2 outer capsid polypeptides of the virion. The loss of these 2 proteins results in an increase in density from 1,36g/ml to 1,40g/ml on CsC1 gradients. The BTV, EHDV and AHSV core particles have an associated double-stranded RNA dependent RNA transcriptase that appears to transcribe mRNA optimally at 28⁰C. It was found, at least in the case of BTV, that this low temperature preference is not an intrinsic characteristic of the transcriptase, but is due to a temperature-dependent inhibition of transcription at high core concentrations.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle