Van der Nest, Magrieta AlettaSteenkamp, Emma TheodoraDe Vos, LieschenWienk, RavenSwart, VelushkaVan den Berg, Noelani2025-02-172025-02-172024Van der Nest, M.A., Steenkamp, E.T., De Vos, L. et al. 2024, 'Complete mitochondrial genome sequence of the white root rot pathogen Dematophora necatrix Xylariaceae: Xylariales)', Mitochondrial DNA Part B Resources, vol. 9, no. 9, 1207-1212. https://DOI.org/10.1080/23802359.2024.2403411.2380-235910.1080/23802359.2024.2403411http://hdl.handle.net/2263/100988DATA AVAILABILITY STATEMENT : The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/. The associated BioProject, BioSample, Genbank, and SRA numbers are PRJNA884201, SAMN31015769, PP377641 and SRR28283158.The mitochondrial genome of Dematophora necatrix is 121,350 base pairs in length with a GþC content of 30.19%. Phylogenetic analysis showed that D. necatrix grouped with other members of the Xylariaceae, with which its mitogenome also shares a broadly similar architecture and gene content. The D. necatrix mitogenome contains 14 protein-coding and 26 tRNA-encoding genes, as well as one copy each of the rnl, rns, rps3 and nat1 genes. However, as much as 80% of this genome is intronic or non-coding. This is likely due to expansions and rearrangements caused by the large number of group I introns and the homing endonucleases and reverse-transcriptases they encode. Our study thus provides a valuable foundation from which to explore the mitochondrion’s role in the biology of D. necatrix, and also serves as a resource for investigating the pathogen’s population biology and general ecology.en© 2024 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License.tRNA gene clustersHoming endonucleasesNacetyltransferaseXylariaceaeSDG-15: Life on landArticle