Schulman, M.L. (Martin)Botha, A.E.Muenscher, Stefanie B.Annandale, Cornelius HenryGuthrie, Alan JohnBertschinger, Hendrik Jan2013-04-172014-01-312013-01Schulman, ML, Botha, AE, Muenscher, SB, Annandale, CH, Guthrie, AJ & Bertschinger, HJ 2013, 'Reversibility of the effects of GnRH-vaccination used to suppress reproductive function in mares', Equine Veterinary Journal, vol. 45, no. 1, pp. 111-113.0425-1644 (print)2042-3306 (online)10.1111/j.2042-3306.2012.00577.x7006535470J-6375-20130000-0001-7729-9918http://hdl.handle.net/2263/21295Reasons for performing study: Active immunisation against gonadotrophin-releasing hormone (GnRH) provides a reversible method for control of oestrous behaviour and fertility in mares. Previous reports failed to demonstrate the interval to resumption of cyclic ovarian activity after GnRH-vaccination. Hypothesis: Administration of the GnRH-vaccine ImprovacÒ1 in a large group of mares of various ages will result in effective, reliably reversible suppression of ovarian activity within a two-year period. Methods: The mares, subdivided into three age categories were vaccinated twice (with a 35 d interval) using ImprovacÒ1 and were monitored via blood samples until Day 720 after initial vaccination for serum progesterone concentration determination by radio-immune assay and anti-GnRH antibody titre by enzyme immuno-assay. Samples were collected until individuals resumed cyclic ovarian activity. Results: All mares showed suppression of cyclic ovarian activity (SPC <1 nmol/l) and 92.2% resumed cyclic activity at Day 720 with a mean interval = 417.8 d (SD = 23.19) and median = 344 d. A significant age effect (P=0.028) on the interval, but not on GnRH-AB titre response, was observed between the youngest (<4 years) and oldest (>11 years) categories. Conclusions: Immunising adult mares of all ages with ImprovacÒ1 resulted in a reversible suppression of cyclic ovarian activity in most mares. An age effect, with the youngest mares showing a longer interval to reversibility was observed.en© 2012 EVJ Ltd. The definite version is available at http://www.evj.co.uk/journals/GnRH-vaccinationHorsesMaresAnoestrusGonadotrophin-releasing hormone (GnRH)TitresReversibilityVaccination of animalsMares -- VaccinationLuteinizing hormone releasing hormonePostprint Article