Polson, A.Van Rooy, P.J.Marais, E.J.Alexander, R.A.Clark, R.Sterne, M.Louw, J.G.Van der Reyden, D.De Kock, V.E.2017-02-022017-02-0220171951Polson, A, Van Rooy, PJ & Marais, EJ 1951, 'A photometric method for quantitative paper partition chromatography of amino acids’, Onderstepoort Journal of Veterinary Research, vol. 25,no. 1, pp. 31-38.0330-2465http://hdl.handle.net/2263/58818The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Due to the difficulties involved in the visual estimation of amino acids in protein hydrolysates from serial dilutions on chromatograms a very simple photoelectric method was devised to facilitate the accurate estimation of amino acid concentration. Sources of errors which must be avoided are temperature fluctuations of the chromatographic tank, non-uniform application of ninhydrin to the filter paper, too high amino acid concentrations and discolorations of the filter paper by organic solvents. The method was applied to the analysis of silk fibroin and values were obtained which agreed well with those determined by previous workers.en© 1951 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © 2017 University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle