Ngoveni, Harry G.Van Schalkwyk, AntoinetteKoekemoer, J.J.O. (Otto)2019-12-022019-12-022019-07-18Ngoveni, H.G., Schalkwyk, A. & Koekemoer, J.J.O. 2019, 'Evidence of intragenic recombination in African horse sickness virus', Viruses, vol. 11, art. 654, pp. 1-15.1999-491510.3390/v11070654http://hdl.handle.net/2263/72446Intragenic recombination has been described in various RNA viruses as a mechanism to increase genetic diversity, resulting in increased virulence, expanded host range, or adaptability to a changing environment. Orbiviruses are no exception to this, with intragenic recombination previously detected in the type species, bluetongue virus (BTV). African horse sickness virus (AHSV) is a double-stranded RNA virus belonging to the Oribivirus genus in the family Reoviridae. Genetic recombination through reassortment has been described inAHSV, but not through homologous intragenic recombination. The influence of the latter on the evolution of AHSV was investigated by analyzing the complete genomes of more than 100 viruses to identify evidence of recombination. Segment-1, segment-6, segment-7, and segment-10 showed evidence of intragenic recombination, yet only one (Segment-10) of these events was manifested in subsequent lineages. The other three hybrid segments were as a result of recombination between field isolates and the vaccine derived live attenuated viruses (ALVs).en© 2019 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.Orbivirus genomeVirus recombinationAfrican horse sickness virus (AHSV)RNA virusesdsRNA virusSerotypesExpressionMechanismIdentificationProteinSouth Africa (SA)Phylogenetic analysisBluetongue virus (BTV)Article