Kyomuhimbo, Hilda DinahFeleni, UsisiphoHaneklaus, Nils H.Brink, Hendrik Gideon2024-10-012024-10-012023-08-21Kyomuhimbo, H.D.; Feleni, U.; Haneklaus, N.H.; Brink, H. Recent Advances in Applications of Oxidases and Peroxidases Polymer-Based Enzyme Biocatalysts in Sensing and Wastewater Treatment: A Review. Polymers 2023, 15, 3492. https://DOI.org/10.3390/polym15163492.2073-436010.3390/polym15163492http://hdl.handle.net/2263/98392DATA AVAILABILITY STATEMENT : Data sharing is not applicable to this article as no new data were created or analyzed in this study.Oxidase and peroxidase enzymes have attracted attention in various biotechnological industries due to their ease of synthesis, wide range of applications, and operation under mild conditions. Their applicability, however, is limited by their poor stability in harsher conditions and their non-reusability. As a result, several approaches such as enzyme engineering, medium engineering, and enzyme immobilization have been used to improve the enzyme properties. Several materials have been used as supports for these enzymes to increase their stability and reusability. This review focusses on the immobilization of oxidase and peroxidase enzymes on metal and metal oxide nanoparticle-polymer composite supports and the different methods used to achieve the immobilization. The application of the enzyme-metal/metal oxide-polymer biocatalysts in biosensing of hydrogen peroxide, glucose, pesticides, and herbicides as well as blood components such as cholesterol, urea, dopamine, and xanthine have been extensively reviewed. The application of the biocatalysts in wastewater treatment through degradation of dyes, pesticides, and other organic compounds has also been discussed.en© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.Enzyme immobilizationNanoparticlesMetal and metal oxide-polymer compositesBiosensorsWastewater treatmentSDG-12: Responsible consumption and productionArticle