Titoy, G.A.P.Verwoerd, Daniel Wynand2013-09-122013-09-1220131995Titoy, GAP 1995, 'An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm)’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 3, pp. 215.0330-2465http://hdl.handle.net/2263/31704The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37°C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0°C in 82,8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69,1% as assessed by motility.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineCryopreservationSheathed third-stage larvaeGaigeria pachyscelisSandveld hookwormVeterinary medicine -- South AfricaArticle