Tan, KenWang, Zheng-WeiLi, HuaYang, Ming-XianPirk, Christian Walter WernerHepburn, H. RandallRadloff, Sarah E.2011-05-042011-05-042010-09Tan, K, Wang, ZW, Li, H, Yang, MX, Pirk, CWW, Hepburn, HR & Radloff, SE 2010, 'Responses of queenright and queenless workers of Apis cerana to 9-keto-2(E)-decenoic acid, a pheromonal constituent of the mandibular gland', Journal of Chemical Ecology, vol. 36, no. 9, pp. 966-968. [http://www.springerlink.com/content/104273/]0098-03311573-1561 (online)10.1007/s10886-010-9833-zhttp://hdl.handle.net/2263/16465In dequeened honeybee colonies ovarian activation occurs in some workers, and the pheromonal bouquets of these laying workers become more queen-like. In the Asiatic honeybee, Apis cerana, we compared the amounts of 9-keto-2(E)-decenoic acid (9-ODA), a mandibular gland pheromone component, between non-laying workers from queenright colonies and laying workers from queenless colonies and, further, applied synthetic 9-ODA to workers to determine whether workers discriminate workers with activated ovaries based on the level of this compound. Levels of 9-ODA were higher in laying workers from dequeened colonies than in non-laying workers from queenright colonies. In both queenright and queenless colonies, more workers treated with 9-ODA were attacked by other workers than were control-treated workers. These results suggest that detection of pseudoqueens in A. cerana is mediated by changes in 9-ODA.en© Springer Science+Business Media, LLC 2010AggressionAsiatic honeybeeLaying workers9-keto-2(E)-decenoic acid9-ODAHoneybee (Apis mellifera)Apis ceranaPheromonesPostprint Article