Wilken, Pieter MarkusSteenkamp, Emma TheodoraHall, Tracy A.De Beer, Z. WilhelmWingfield, Michael J.Wingfield, Brenda D.2012-02-272012-02-272012Wilken, PM, Steenkamp, ET, Hall, TA, De Beer, ZW, Wingfield, MJ & Wingfield, BD, Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes, Fungal Biology (2012), doi: 10.1016/j.funbio.2012.01.0021878-6146 (print)1878-6162 (online)10.1016/j.funbio.2012.01.002http://hdl.handle.net/2263/18241In heterothallic Ascomycota, two opposite but distinct mating types control all sexual processes. Using mating crosses, mating types were assigned to ten isolates of the heterothallic fungal species Ophiostoma quercus. Primers were subsequently designed to target the MAT1-1-1, MAT1-1-3 (of the mating type 1 idiomorph), and MAT1-2-1 (of the mating type 2 idiomorph) genes in these isolates. Results showed that all isolates contained the full gene sequence for the MAT1-2-1 gene. In addition, fragments of the MAT1-1-1 and MAT1-1-3 genes were sequenced from all isolates. These results were unexpected, as each isolate from a heterothallic species would typically contain only one of the two possible MAT idiomorphs.en© 2012 The British Mycological Society. Published by Elsevier Ltd All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Fungal Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Fungal Biology, 2012, doi: 10.1016/j.funbio.2012.01.002Fungal matingMAT1-1MAT1-2Heterothallic fungusMating systemOphiostoma -- GeneticsAscomycetes -- GeneticsPostprint Article