Jacobs, Paul JuanMcKechnie, Andrew E.2014-11-032014-09Jacobs, PJ & McKechnie, AE 2014, 'Experimental sources of variation in avian energetics : estimated basal metabolic rate decreases with successive measurements', Physiological and Biochemical Zoology, vol. 87, no. 5, pp. 762-769.1522-2152 (print)1537-5293 (online)10.1086/676933http://hdl.handle.net/2263/42451Basal metabolic rate (BMR) is one of the most widely used metabolic variables in endotherm ecological and evolutionary physiology. Surprisingly few studies have investigated howBMR is influenced by experimental and analytical variables over and above the standardized conditions required for minimum normothermic resting metabolism. We tested whether avian BMR is affected by habituation to the conditions experienced during laboratory gas exchange measurements by measuring BMR five times in succession in budgerigars (Melopsittacus undulatus) housed under constant temperature and photoperiod. Both the magnitude and the variability of BMR decreased significantly with repeated measurements, from 0.410 0.092 W (n p 9) during the first measurement to 0.285 0.042 W (n p 9) during the fifth measurement. Thus, estimated BMR decreased by ∼30% within individuals solely on account of the number of times they had previously experienced the experimental conditions. The most likely explanation for these results is an attenuation with repeated exposure of the acute stress response induced by birds being handled and placed in respirometry chambers. Our data suggest that habituation to experimental conditions is potentially an important determinant of observed BMR, and this source of variation needs to be taken into account in future studies of metabolic variation among individuals, populations, and species.en© 2014 by The University of Chicago. All rights reserved.Body temperatureHabituationMetabolic rateRespirometrySampling intervalArticle