May, Catherine EdithGuthrie, Alan JohnSchulman, M.L. (Martin)2020-02-282020-02-282019-09May, C.E., Guthrie, A.J. & Schulman, M.L. 2019, 'Direct culture–independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses', Journal of Veterinary Diagnostic Investigation, vol. 31, no. 5, pp. 792-794.1040-6387 (print)1943-4936 (online)10.1177/1040638719871089http://hdl.handle.net/2263/73609We report herein the use of crude extracts obtained from samples of Taylorella equigenitalis–infected horses for the purpose of multi-locus sequence typing (MLST). Samples (n = 36) were collected from horses in South Africa from 1996 to 2017: 34 from genital swabs (stored at −20°C for 2–3 y) and 2 from cryopreserved raw semen aliquots (stored at −70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis.en© 2019 The Author(s)Contagious equine metritis (CEM)Direct culture–independent strain typingTaylorella equigenitalisHorse (Equus caballus)South Africa (SA)Frozen (cryopreserved) semenHorse breedingBiosecurityVeterinary science articles SDG-03Veterinary science articles SDG-09Veterinary science articles SDG-12Veterinary science articles SDG-15Veterinary science articles SDG-17Postprint Article