Davies, Kalina T.J.Tsagkogeorga, GeorgiaBennett, Nigel CharlesDavalos, Liliana M.Faulkes, Christopher G.Rossiter, Stephen J.2014-10-032014-10-032014-10Davies, KTJ, Tsagkogeorga, G, Bennett, NC, Dávalos, LM, Faulkes, CG & Rossiter, SJ 2014, 'Molecular evolution of growth hormone and insulin-like growth factor 1 receptors in long-lived, small-bodied mammals', Gene, vol. 549, no. 2, pp. 228-236.0378-1119 (print)1879-0038 (online)10.1016/j.gene.2014.07.061http://hdl.handle.net/2263/42228Mammals typically display a robust positive relationship between lifespan and body size. Two groups that deviate markedly from this pattern are bats and African mole-rats, with members of both groups being extremely long-lived given their body size, with the maximum documented lifespan for many species exceeding 20 years. A recent genomics study of the exceptionally long-lived Brandt's bat, Myotis brandtii (41 years), suggested that its longevity and small body size may be at least partly attributed to key amino acid substitutions in the transmembrane domains of the receptors of growth hormone (GH) and insulin-like growth factor 1 (IGF1). However, whereas elevated longevity is likely to be common across all 19 bat families, the reported amino acid substitutionswere only observed in two closely related bat families. To test the hypothesis that an altered GH/IGF1 axis relates to the longevity of African mole-rats and bats,we compared and analysed the homologous coding gene sequences in genomic and transcriptomic data from 26 bat species, five mole-rats and 38 outgroup species. Phylogenetic analyses of both genes recovered themajority of nodes in the currently accepted species tree with high support. Compared to other clades, such as primates and carnivores, the bats and rodents had longer branch lengths. The single 24 amino acid transmembrane domain of IGF1Rwas found to bemore conserved across mammals compared to that of GHR.Within bats, considerable variation in the transmembrane domain of GHR was found, including a previously unreported deletion in Emballonuridae. The transmembrane domains of rodents were found to be more conserved, with mole-rats lacking uniquely conserved amino acid substitutions. Molecular evolutionary analyses showed that both genes were under purifying selection in bats andmole-rats. Our findings suggest thatwhile the previously documentedmutations may confer some additional lifespan to Myotis bats, other, as yet unknown, genetic differences are likely to account for the long lifespans observed in many bat and mole-rat species.en© 2014 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Gene. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Gene, vol. 549, no. 2, pp. 228-236, 2014. doi : 10.1016/j.gene.2014.07.061.MammalsBatsMole-ratsLongevityTransmembrane domainsPostprint Article