Bragg, Robert RichardCoetzee, L.Verwoerd, Daniel Wynand2014-01-272014-01-2720131993Bragg, R.R., Coetzee, L. & Verschoor, J.A. 1993, 'Plasmid-encoded NAD independence in some South African isolates of Haemophilus paragallinarum’, Onderstepoort Journal of Veterinary Research, vol. 60, no. 2, pp. 147-152.0330-2465http://hdl.handle.net/2263/33103The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Atypical Haemophilus paragallinarum have been isolated from both laying hens and broilers suffering from typical symptoms of infectious coryza in South Africa. Re-inoculation of these bacteria into SPF chickens resulted in similar pathology. The bacteria could be successfully re-isolated from the experimentally infected chickens. Four of the isolates from layers and 3 of those from broilers were found to be closely related to H. paragallinarum serotype A (0083 strain) when tested by the use of a panel of locally developed monoclonal antibodies in the enzyme linked immunosorbent assay (ELISA) . A total of 15 isolates from layers and 19 from broilers were found to be more typical of previously collected South African field isolates of H. paragallinarum. A 3rd group, consisting of 5 isolates from layers and 15 from broilers, showed no reaction with the panel of monoclonal antibodies. All the isolates were regarded as atypical because they no longer required V factor (NAD) for growth, whereas strain 0083 and previously collected field isolates M 85 and SB 86 did require it.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineChickensVeterinary medicine -- South AfricaArticle