Mason, J.H.Scheuber, J.R.Du Toit, P.J.2017-05-222017-05-2220171936Mason, JH & Scheuber, JR 1936, 'The production of immunity to Cl. chauvoei’, Onderstepoort Journal of Veterinary Science and Animal Industry, vol. 7, no. 1, pp. 143-165.0330-2465http://hdl.handle.net/2263/60592The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.1. Potent Cl. chauvoei vaccine (anaculture) may be made in horse-flesh infusion broth plus 1.0 per cent. peptone, 0.85 per cent. salt and 2-5 per cent. of serum and/or the haemolysed red cells of the horse, ox and sheep (meat particles one-third by volume). 2. Tryptic digests of horse muscle media produce satisfactory vaccines. 3. Boiled, dense suspensions of Cl. chauvoei injected subcutaneously into sheep produce a high degree of protection to culture injected intramuscularly. 4. The medium and method finally decided on as a routine measure was: ox-liver infusion broth (liver particles one-third by volume) plus 1.0 per cent. peptone, 0.85 per cent. salt and 1 per cent. glucose added after sterilization, pH 7.8, 36 hours’ incubation, and 0.5 per cent. formolization for three days at 37°C.en© 1936 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © 2017 University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle