Stoltz, M.A.Van der Merwe, Christiaan F.Coetzee, J.Verwoerd, Daniel Wynand2013-08-152013-08-1520131996Stoltz, MA, Van der Merwe, CF, Coetzee, J & Huismans, H 1996, 'Subcellular localization of the nonstructural protein NS3 of African horsesickness virus’. Onderstepoort Journal of Veterinary Research, vol. 63, no. 1, pp. 57-61.0330-2465http://hdl.handle.net/2263/22034The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The subcellular localization of the minor nonstructural protein NS3 of African horsesickness virus (AHSV) has been investigated by means of immunogold electron-microscopical analysis. NS3 was observed in perturbed regions of the plasma membrane of AHSV-infected VERO cells, and its presence appears to be associated with events of viral release. These events are budding, whereby released viruses acquire fragments from the host-cell membrane, as well as by the extrusion of nonenveloped particles through the cell membrane. The membrane association of NS3 was confirmed by its detection in the disrupted plasma membranes of cells infected with an NS3 baculovirus recombinant. The absence of NS3 on intact cell membranes suggests that the protein is not exposed extracellularly.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineAfrican horsesickness virus (AHSV)Non-structural protein 3 (NS3)NS3Subcellular localizationVeterinary medicine -- South AfricaAfrican horse sickness -- South AfricaArticle