Khasapane, Ntelekwane GeorgeKwenda, StanfordKhumalo, Zamantungwa Thobeka HappinessNkhebenyane, Sebolelo JaneThekisoe, Oriel2024-08-232024-08-232023-12Khasapane, N.G., Kwenda, S., Khumalo, Z.T.H. et al. 2023, 'Three novel sequencing types from seventeen staphylococcus aureus genomes isolated from dairy cows milk in the Free State Province of South Africa', Microbiology Resource Announcements, vol. 12, no. 12, pp. 1-4. DOI: 10.1128/MRA.00739-23.2576-098X (online)10.1128/MRA.00739-23http://hdl.handle.net/2263/97843DATA AVAILABILITY : This whole-genome sequence project has been registered in DDBJ/ENA/GenBank with the BioProject accession number PRJNA981445. The Genome submission numbers, BioSample accession numbers, and PubMLST isolate IDs are provided in Table 1.Staphylococcus aureus is one of the major pathogens causing bovine mastitis, which results in huge economic losses in the dairy industry worldwide. Here, we report genome sequences of 17 S. aureus strains, with three novel sequencing types (ST8495, ST8500, and ST8501) isolated from the milk of dairy cows with subclinical mastitis.en© 2023 Khasapane et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.BovineSubclinical mastitisStaphylococcus aureusSequencing typesWhole genome sequencing (WGS)Dairy cattleSDG-03: Good health and well-beingSDG-02: Zero hungerFree State Province, South AfricaArticle