Da Costa Mendes, Vasco ManuelDe Beer, M.C.Goosen, G.H.Steele, Andrew DuncanVerwoerd, Daniel Wynand2014-01-222014-01-2220131994Da Costa Mendes, VM, De Beer, MC, Goosen, GH & Steele, AD 1994, 'Isolation and preliminary characterization of a caprine rotavirus’, Onderstepoort Journal of Veterinary Research, vol. 61, no. 4, pp. 291-294.0330-2465http://hdl.handle.net/2263/33060The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Five cytopathic rotavirus strains were isolated in MA 104 cells from stool specimens of kids with diarrhoea. Pre-treatment of the virus with trypsin and incorporation of low levels of trypsin in the maintenance medium were important for the successful cultivation of the strains in these cells. The isolates were shown to be group A rotaviruses by antigenic reactivity with a group A monoclonal antibody. This was confirmed by the migration patterns of the viral RNA genome during polyacrylamide gel electrophoresis, which also confirmed that all five strains had an identical RNA electropherotype. Analysis with monoclonal antibodies to the subgroup-specific VP6 antigen showed that these strains carried the subgroup I epitope.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle