Van der Merwe, Nicolaas Albertus (Albie)Phakalatsane, TshiamoWilken, Pieter Markus2024-06-282024-06-282023-05-26van der Merwe, N.A.; Phakalatsane, T.; Wilken, P.M. The Unique Homothallic Mating-Type Loci of the Fungal Tree Pathogens Chrysoporthe syzygiicola and Chrysoporthe zambiensis from Africa. Genes 2023, 14, 1158. https://DOI.org/10.3390/genes14061158.2073-4425 (online)10.3390/genes14061158http://hdl.handle.net/2263/96733DATA AVAILABILITY STATEMENT : Draft genome sequences generated during this study were deposited at the NCBI under BioProject PRJNA971112 https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA9 71112 (accessed on 19 May 2023).Chrysoporthe syzygiicola and C. zambiensis are ascomycete tree pathogens first described from Zambia, causing stem canker on Syzygium guineense and Eucalyptus grandis, respectively. The taxonomic descriptions of these two species were based on their anamorphic states, as no sexual states are known. The main purpose of this work was to use whole genome sequences to identify and define the mating-type (MAT1) loci of these two species. The unique MAT1 loci for C. zambiensis and C. syzygiicola consist of the MAT1-1-1, MAT1-1-2, and MAT1-2-1 genes, but the MAT1-1-3 gene is absent. Genes canonically associated with opposite mating types were present at the single mating-type locus, suggesting that C. zambiensis and C. syzygiicola have homothallic mating systems.en© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.ChrysoportheAscomyceteMating-typeHomothallicSDG-15: Life on landArticle