Halstead, S.K.Gourlay, D.S.Penderis, J.Bianchi, E.Dondi, M.Wessmann, A.Musteata, M.Le Chevoir, M.Martinez-Anton, L.Bhatti, S.F.M.Volk, H.Mateo, I.Tipold, A.Ives, E.Pakozdy, A.Gutierrez-Quintana, R.Brocal, J.Whitehead, Z.Granger, N.Pazzi, PaoloHarcourt-Brown, T.Jose-Lopez, R.Rupp, S.Schenk, H.C.Smith, P.Gandini, G.Menchetti, M.Mortera-Balsa, V.Rusbridge, C.Tauro, A.Cozzi, F.Deutschland, M.Tirrito, F.Freeman, P.Lowrie, M.Jackson, M.R.Willison, H.J.Rupp, A.2022-12-152022-12-152022-02Halstead, S.K., Gourlay, D.S., Penderis, J. et al. (2022), Serum anti-GM2 and anti-GalNAc-GD1a IgG antibodies are biomarkers for acute canine polyradiculoneuritis. Journal of Small Animal Practice, 63: 104-112. https://doi.org/10.1111/jsap.13439.0022-4510 (print)1748-5827 (online)10.1111/jsap.13439https://repository.up.ac.za/handle/2263/88836OBJECTIVES: A previous single-country pilot study indicated serum anti-GM2 and anti-GA1 anti-glycolipid antibodies as potential biomarkers for acute canine polyradiculoneuritis. This study aims to validate these findings in a large geographically heterogenous cohort. MATERIALS AND METHODS: Sera from 175 dogs clinically diagnosed with acute canine polyradiculoneuritis, 112 dogs with other peripheral nerve, cranial nerve or neuromuscular disorders and 226 neurologically normal dogs were screened for anti-glycolipid antibodies against 11 common glycolipid targets to determine the immunoglobulin G anti-glycolipid antibodies with the highest combined sensitivity and specificity for acute canine polyradiculoneuritis. RESULTS: Anti-GM2 anti-glycolipid antibodies reached the highest combined sensitivity and specificity (sensitivity: 65.1%, 95% confidence interval 57.6 to 72.2%; specificity: 90.2%, 95% confidence interval 83.1 to 95.0%), followed by anti-GalNAc-GD1a anti-glycolipid antibodies (sensitivity: 61.7%, 95% confidence interval 54.1 to 68.9%; specificity: 89.3%, 95% confidence interval 82.0 to 94.3%) and these anti-glycolipid antibodies were frequently present concomitantly. Anti-GA1 anti-glycolipid antibodies were detected in both acute canine polyradiculoneuritis and control animals. Both for anti-GM2 and anti-GalNAc-GD1a anti-glycolipid antibodies, sex was found a significantly associated factor with a female to male odds ratio of 2.55 (1.27 to 5.31) and 3.00 (1.22 to 7.89), respectively. Anti-GalNAc-GD1a anti-glycolipid antibodies were more commonly observed in dogs unable to walk (OR 4.56, 1.56 to 14.87). CLINICAL SIGNIFICANCE: Anti-GM2 and anti-GalNAc-GD1a immunoglobulin G anti-glycolipid antibodies represent serum biomarkers for acute canine polyradiculoneuritis.en© 2021 The Authors. Journal of Small Animal Practice published by John Wiley & Sons Ltd on behalf of British Small Animal Veterinary Association. This is an open access article under the terms of the Creative Commons Attribution License.Anti-glycolipid antibodiesSerum anti-GM2Acute canine polyradiculoneuritis (ACP)AntibodiesAnti-glycolipidArticle