Worthington, R.W.Mulders, M.S.G.Bigalke, R.D.Morren, A.J.Verster, Anna J.M.Verwoerd, Daniel WynandWalker, Jane B.Cameron, Colin McKenzieGilchrist, Frances M.C.Steyn, P.J.J.2016-07-062016-07-0620161979Worthington, RW & Mulders, MSG 1979, 'Isolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serum’, Onderstepoort Journal of Veterinary Research, vol. 46, no. 3, pp. 121-124.0330-2465http://hdl.handle.net/2263/53829This article has been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-Format.Antibodies against epsilon toxin were isolated from hyperimmune horse serum by affinity chromatography. Purified epsilon prototoxin covalently bound to Affigel 202 was used as immunosorbent, and antibodies were eluted with 6,0 M guanidine chloride. In a single run 80 mg of antibody could be recovered from a 20 mℓ column of immunosorbent. The antibody was shown to belong to the IgG(T) class of immunoglobulins.en©1979 ARC- Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original) ©2016 University of Pretoria Department of Library Services(digital)Veterinary medicineVeterinary medicine -- South AfricaIsolation and characterization of antibodies to Clostridium perfringens epsilon toxin from hyperimmune horse serumArticle