Taylor, JanetAnyango, Joseph OchiengPotgieter, MarnieKallmeyer, KarlienNaidoo, VinnyPepper, Michael SeanTaylor, J.R.N. (John Reginald Nuttall)2015-08-032015-08-032015-08Taylor, J, Anyango, JO, Potgieter, M, Kallmeyer, K, Naidoo, V, Pepper, MS & Taylor, JRN 2015, 'Biocompatibility and biodegradation of protein microparticle and film scaffolds made from kafirin (sorghum prolamin protein) subcutaneously implanted in rodent model', Journal of Biomedical Materials Research Part A, vol. 103, no. 8, pp. 2582-2590.1549-3296 (print)1552-4965 (online)10.1002/jbm.a.35394A-1508-2008 8621439700http://hdl.handle.net/2263/49223Kafirin, the sorghum prolamin protein, like its maize homologue zein, can be made into micropatticles and films and potentially used as a biomaterial. Zein has good bio- and cytocompatibility. Kafirin could be advantageous as it is more hydrophobic, more cross-linked, more slowly digested by mammalian proteases than zein and is non-allergenic. The safety and biocompatibility of kafirin implants in two forms was determined in rodent models. One week post subcutaneous injection of kafirin micropatticles (size S[!m diameter) in mice, chronic inflammation, abnormal red blood cells and gross fibrin formation were observed. This chronic inflammatory response was possibly caused by the release of hydrolysis products such as glutamate during the degradation of the kafirin microparticles. In contrast, films made from kaftrin microparticles (50 [!ill thickness, folded into I cm3 ) implanted in rats showed no abnormal inflammatory reactions and were only partially degraded by day 28. The slower degradation of the kafirin films was probably due to their far smaller surface area when compared to kafirin micropatticles. Thus, kafirin films appear to have potential as a biomaterial. This study also raises awareness that the form of prolamin based biomaterials, (kafirin and zein) should be considered when assessing the safety of such materials.en© 2014 Wiley Periodicals, Inc.. This is the pre-peer reviewed version of the following article : Biocompatibility and biodegradation of protein microparticle and film scaffolds made from kafirin (sorghum prolamin protein) subcutaneously implanted in rodent models in Journal of Biomedical Materials Research Part A, vol. 103, no. 8, pp. 2582-2590, 2015. doi : 10.1002/jbm.a.35394. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1002/(ISSN)1552-4965.MicroparticleKafirinHistologyFilmBiocompatibilityPostprint Article