Lall, NamritaKishore, NavneetBinneman, BrigitteTwilley, DanielleVan de Venter, MarynaDu Plessis-Stoman, DebbieBoukes, GerhardtHussein, Ahmed A.2015-03-232015-03-232015Lall, N, Kishore, N, Binneman, B, Twilley, D, Van de Venter, M, Plessis-Stoman, DD, Boukes, G & Hussein, A 2015, 'Cytotoxicity of syringin and 4-methoxycinnamyl alcohol isolated from Foeniculum vulgare on selected human cell lines', Natural Product Research, vol. 29, no. 18, pp. 1752-1756.1478-6419 (print)1478-6427 (online)10.1080/14786419.2014.999058http://hdl.handle.net/2263/44108The present study was carried out to determine the cytotoxic effect of seven plant extracts and the isolated compounds; syringin and 4-methoxycinnamyl alcohol on cancerous and non-cancerous cells. The ethanol extract of F. vulgare was found to exhibit the most significant toxicity with an IC50 value of 19.97 μg/mL on HeLa cells. Bioassay guided fractionation lead to the isolation of two compounds, syringin (1) and 4-methoxycinnamyl alcohol (2). Both compounds showed toxicity against MCF-7, HeLa, and DU145 cancer cell line. The results showed that compound 2 showed high toxicity against all the cancer cell lines with IC50 values of 14.24, 7.82 and 22.10 μg/mL, respectively. 4-Methoxycinnamyl alcohol also showed no apoptotic effect in cell cycle analysis after 48 hours at a concentration of 10 μg/mL. However DNA fragmentation study revealed that, necrosis took place at a concentration of 10 μg/mL after 48 h exposure.en© Taylor and Francis. This is an electronic version of an article published in Natural Product Research, vol. 29, no. 18, pp. 1752-1756, 2015. doi : 10.1080/14786419.2014.999058. Natural Product Research is available online at : http://www.tandfonline.comloi/gnpl20.Foeniculum vulgareCytotoxicity4-Methoxycinnamyl alcoholCell cycle analysisDNA fragmentationPostprint Article