Contrafatto, G.Pammenter, M.D.Bigalke, R.D.2014-10-152014-10-1520141988Contrafatto, G & Pammenter, MD 1988, 'Immunization procedure-related immunoglobulin levels in the development of antibodies against Cysticercus cellulosae', Onderstepoort Journal of Veterinary Research, vol. 55, no. 3, pp. 169-172.0330-2465http://hdl.handle.net/2263/42380The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Various immunization procedures were investigated in an effort to improve the number of hybridomas producing antibodies against Cysticercus cellulosae. Ten groups of 5 BALB/c mice were subjected to different immunization procedures and were bled repeatedly over a period of 68 days. The samples of sera thus obtained were tested by enzyme linked immunosorbent assay : total immunoglobulins, lgG and IgM levels were determined. In general, total anticyst antibody titres increased during the course of immunization but in 3 groups the final titre was lower than the maximal antibody titre. Overall, immune tolerance did not appear to be a problem and longer immunization programs seemed to end with slightly higher antibody levels. So far, 4 mice from the group that exhibited the highest immunoglobulin levels have been used for hybridoma production. Out of 124 hybridomas thus obtained, only 1 secreted antibodies against Cysticercus cellulosae.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle