Maharaj, KarishmaBradfield, M.F.A. (Michael Ford Alexander)Nicol, Willie2015-08-172015-08-172014-09Maharaj, K, Bradfield, MFA & Nicol, W 2014, 'Succinic acid-producing biofilms of Actinobacillus succinogenes : reproducibility, stability and productivity', Applied Microbiology and Biotechnology, vol. 98, no.17, pp. 7379-7386.0175-7598 (print)1432-0614 (online)10.1007/s00253-014-5779-3http://hdl.handle.net/2263/49355Continuous anaerobic fermentations were performed in a biofilm reactor packed with Poraver® beads. Dilution rates (D) varied between 0.054 and 0.72 h−1, and D-glucose and CO2 gas were used as carbon substrates. Steady-state conditions were shown to be repeatable and independent of the operational history. Production stability was achieved over periods exceeding 80 h at values of D below 0.32 h−1. In these situations, steady-state variation (expressed as fluctuations in NaOH neutralisation flow rates) exhibited a standard deviation of less than 5 % while no indication of biofilm deactivation was detected. The total biomass amount was found to be independent of the dilution rate with an average dry concentration of 23.8± 2.9 g L−1 obtained for all runs. This suggests that the attachment area controls the extent of biofilm accumulation. Specific succinic acid (SA) productivities, based on the total biomass amount, exhibited a substantial decrease with decreasing D. An SA volumetric productivity of 10.8 g L−1 h−1 was obtained at D=0.7 h−1—the highest value reported to date in Actinobacillus succinogenes fermentations. SA yields on glucose increased with decreasing D, with a yield of 0.90±0.01 g g−1 obtained at a D of 0.054 h−1. Production of formic acid approached zero with decreasing D, while the succinic to acetic acid ratio increased with decreasing D, resulting in an increasing SA yield on glucose.en© Springer-Verlag Berlin Heidelberg 2014. The original publication is available at : http://link.springer.comjournal/253.Actinobacillus succinogenesBiofilm reactorContinuous fermentationProductivityStabilitySuccinic acidPostprint Article