Wahadoszamen, Md.Kruger, T.P.J. (Tjaart)Ara, Anjue ManeVan Grondelle, RienkGwizdala, Michal2020-10-192020-07Wahadoszamena, M., Kruger, T.P.J., Ara, A.M. et al. 2020, 'Charge transfer states in phycobilisomes', Biochimica et Biophysica Acta (BBA) - Bioenergetics, vol. 1861, no 7, art. 148187, pp. 1-10.0005-272810.1016/j.bbabio.2020.148187http://hdl.handle.net/2263/76539Phycobilisomes (PBs) absorb light and supply downstream photosynthetic processes with excitation energy in many cyanobacteria and algae. In response to a sudden increase in light intensity, excess excitation energy is photoprotectively dissipated in PBs by means of the orange carotenoid protein (OCP)-related mechanism or via a light-activated intrinsic decay channel. Recently, we have identified that both mechanisms are associated with far-red emission states. Here, we investigate the far-red states involved with the light-induced intrinsic mechanism by exploring the energy landscape and electro-optical properties of the pigments in PBs. While Stark spectroscopy showed that the far-red states in PBs exhibit a strong charge-transfer (CT) character at cryogenic temperatures, single molecule spectroscopy revealed that CT states should also be present at room temperature. Owing to the strong environmental sensitivity of CT states, the knowledge gained from this study may contribute to the design of a new generation of fluorescence markers.en© 2020 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication inBiochimica et Biophysica Acta - Bioenergetics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Biochimica et Biophysica Acta - Bioenergetics, vol. 1861, no 7, art. 148187, pp. 1-10, 2020. doi : 10.1016/j.bbabio.2020.148187.Light harvestingExcitation energy flow regulationSingle molecule spectroscopyStark spectroscopyPhotosynthesisPhycobilisomesPostprint Article