De Villiers, SaramarieVan der Walt, J.G.Procos, J.Bigalke, R.D.Cameron, Colin McKenzieGilchrist, Frances M.C.Morren, A.J.Verster, Anna J.M.Verwoerd, Daniel WynandWalker, Jane B.De Kock, V.E.2016-07-062016-07-0620161977De Villiers, S, Van der Walt, JG & Procos, J 1977, 'An accurate, sensitive and reproducible method for colorimetric estimation of free fatty acids in plasma’, Onderstepoort Journal of Veterinary Research, vol. 44, no. 3, pp. 169-172.0330-2465http://hdl.handle.net/2263/53722The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A sensitive and reproducible method permitting the estimation of 0,030-3,000 mM of free fatty acids (FFA) in plasma with a standard error of 0,006 5 was developed. The method is based on the extraction of phosphate-buffered plasma with a chloroform-heptane-methanol mixture, formation of the cobalt complex and on the subsequent determination of the metal with 1-nitroso-2 naphthol. The method was used to obtain normal values (mean 0,547 mM range 0,147-1,197 mM) for Merino wethers fed lucerne hay.en© 1977 ARC – Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © 2016 University of Pretoria. Department of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle